Journal:

Article Title: Inhibition of Rac GTPase triggers a c-Jun- and Bim-dependent mitochondrial apoptotic cascade in cerebellar granule neurons

doi: 10.1111/j.1471-4159.2005.03252.x

Figure Lengend Snippet: ToxB induces apoptosis and elicits a selective caspase-mediated degradation of Rac1 GTPase in CGNs. (a) CGNs were incubated for 24 h in complete medium (containing serum and 25 mm KCl) in the presence of either vehicle (Veh; 2 μg/mL BSA in PBS) or ToxB (40 ng/mL). Following incubation, cells were fixed in paraformaldehyde and nuclei were stained with Hoechst dye. CGNs exposed to ToxB showed marked nuclear condensation and fragmentation characteristic of apoptosis. Scale bar, 10 μm. (b) Representative immunoblots of lysates obtained from CGNs incubated as described in (a). The blots shown were obtained from the BD PowerBlot™ (see Experimental procedures for details) using specific monoclonal antibodies against Rac1 (upper blots), RhoA (middle blots), or Cdc42 (lower blots). The immunoreactive Rho family GTPases are indicated by the arrows. Other protein bands apparent on the membranes represent adjacent lanes of the PowerBlot™ that were probed for distinct proteins and are shown to give an indication of the overall equality of protein loading. (c) The fold change in protein content induced by 24 h of ToxB treatment for each of the Rho family members blotted for in (b) is shown. Data represent the means ± range of duplicate PowerBlot™ comparisons for each GTPase. The dotted lines indicate no change (-1 to +1 fold). (d) CGNs were exposed for 8 h to ToxB in the absence or presence of either a pan-caspase inhibitor (Boc-Asp(OMe)-FMK; 200 μm) or zVAD-FMK; 200 μm) or a proteasome inhibitor (MG132; 10 μm). Following incubation, cell lysates were immunoblotted for Rac1.

Article Snippet: The blots shown were obtained from the BD PowerBlot™ (see Experimental procedures for details) using specific monoclonal antibodies against Rac1 (upper blots), RhoA (middle blots), or Cdc42 (lower blots).

Techniques: Incubation, Staining, Western Blot

Journal: Channels (Austin, Tex.)

Article Title: The deubiquitinating enzyme USP10 regulates the endocytic recycling of CFTR in airway epithelial cells.

doi: 10.4161/chan.4.3.11223

Figure Lengend Snippet: Figure 2. USP10 immunoprecipitates CFTR. HBE cells were lysed, USP10 was immuno- precipitated using an anti-USP10 antibody, and western blot analysis was performed for USP10 and CFTR. LYSATE, cell lysates (2.5% of lysate run on gel). USP10 IP indicates proteins that were immunoprecipitated using the USP10 antibody. The non-immune IgG did not immunoprecipitate USP10 or CFTR, and thus served as a negative control. Experiments were performed three times. Representative blots are shown.

Article Snippet: To determine if CFTR interacts with USP10 in early endosomal fractions, USP10 was immunoprecipitated from early endosomal fractions isolated from the HBE cell lysate by methods described previously in detail.30 USP10 was immunoprecipitated by incubation with 5 μg of a polyclonal USP10 antibody (Bethyl Laboratories) and protein A-agarose complex.

Techniques: Western Blot, Immunoprecipitation, Negative Control

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: Features of Individual 1, with the p.Leu610Pro DDR2 Variant

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques: Variant Assay

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: Overview of Phenotypic Features

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques: Variant Assay

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: DDR2 Structure and Amino Acid Conservation

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques:

Journal: American Journal of Human Genetics

Article Title: Recurrent, Activating Variants in the Receptor Tyrosine Kinase DDR2 Cause Warburg-Cinotti Syndrome

doi: 10.1016/j.ajhg.2018.10.013

Figure Lengend Snippet: Autophosphorylation of DDR2 and Effect of Dasatinib Treatment

Article Snippet: For immunoblot analyses, cells were starved of serum overnight before being harvested, separated on a high-resolution gel system, transferred to nitrocellulose membranes, and incubated overnight at 4°C with antibodies against phospho-Tyr740-DDR2 (#MAB25382) and DDR2 (#MAB2538) (R&D Systems, detailed description in Supplemental Methods ).

Techniques: